ADI-905-708-100

antibody from Enzo Life Sciences

Targeting: CNR1 CANN6, CB-R, CB1, CB1A, CB1K5, CNR

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ADI-905-708-100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-905-708-100, RRID:AB_2038917

Product name

Cannabinoid receptor CB1 polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide affinity purified.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Vial size

100 μg

Storage

-20°C

Handling

Keep on ice at all times.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis: MW Marker (1) and 10µg of rat brain membrane extract (2) probed with Cannabinoid Receptor (CB1) pAb at 2.8µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 µM concentrations of agonist (HU210) and Inverse agonist (SR141716A/Hemopressin) and probed with Cannabinoid Receptor (CB1) pAb (1:1000 of a 1µg/µl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Membrane ELISA: Membranes (5µg) from rat striatum were treated with indicated concentrations of agonists (HU-210) and inverse agonist (SR141716A) and probed with Cannabinoid Receptor (CB1) pAb by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).