PA5-80123

antibody from Invitrogen Antibodies

Targeting: THBS2 TSP2

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80123

Provider

Invitrogen Antibodies

Product name

Thrombospondin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Tsp2 Facilitates Tumor-associated Fibroblasts Formation and Promotes Tumor Progression in Retroperitoneal Liposarcoma.

Xu C, Yan L, Guan X, Wang Z, Wu J, Lv A, Liu D, Liu F, Dong B, Zhao M, Jia L, Tian X, Hao C
International journal of biological sciences 2022;18(13):5038-5055

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MicroRNA‑203a‑3p is a candidate tumor suppressor that targets thrombospondin 2 in colorectal carcinoma.

Qian Z, Gong L, Mou Y, Han Y, Zheng S
Oncology reports 2019 Nov;42(5):1825-1832

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of Thrombospondin 2 in U-87MG cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Thrombospondin 2 Polyclonal Antibody (Product # PA5-80123) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Tsp2 downregulation suppressed xenograft tumor growth in NOD/SCID mice and the potential mechanism was elucidated by protein microarray. (A) On day 60, the volume of xenograft tumors formed by SW872 shTsp2 cells were smaller than tumors formed by SW872 shCtrl cells (p