- PA5-80123 - Invitrogen Antibodies THBS2 antibody

Submitted references Tsp2 Facilitates Tumor-associated Fibroblasts Formation and Promotes Tumor Progression in Retroperitoneal Liposarcoma.

Xu C, Yan L, Guan X, Wang Z, Wu J, Lv A, Liu D, Liu F, Dong B, Zhao M, Jia L, Tian X, Hao C
International journal of biological sciences 2022;18(13):5038-5055

MicroRNA‑203a‑3p is a candidate tumor suppressor that targets thrombospondin 2 in colorectal carcinoma.

Qian Z, Gong L, Mou Y, Han Y, Zheng S
Oncology reports 2019 Nov;42(5):1825-1832

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Thrombospondin 2 in Lane 1: SMMC whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HT1080 whole cell lysate, Lane 4: JURKAT whole cell lysate, Lane 5: NIH 3T3 whole cell lysate using 40 µg per well. Sample was incubated with Thrombospondin 2 (Product # PA5-80123) at a dilution of 0.5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Thrombospondin 2/THBS2 in, Lane 1: human Hela whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Caco-2 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with Thrombospondin 2 Polyclonal Antibody (Product # PA5-80123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Thrombospondin 2/THBS2 in, Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with Thrombospondin 2 Polyclonal Antibody (Product # PA5-80123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Thrombospondin 2 on paraffin-embedded human placenta tissue. Sample was incubated with Thrombospondin 2 polyclonal antibody (Product# PA5-80123).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow Cytometry of Thrombospondin 2 in U-87MG cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Thrombospondin 2 Polyclonal Antibody (Product # PA5-80123) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Tsp2 downregulation suppressed xenograft tumor growth in NOD/SCID mice and the potential mechanism was elucidated by protein microarray. (A) On day 60, the volume of xenograft tumors formed by SW872 shTsp2 cells were smaller than tumors formed by SW872 shCtrl cells (p

PA5-80123

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