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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-46937 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thrombospondin 2 Recombinant Rabbit Monoclonal Antibody (HL1140)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Store as concentrated solution. Centrifuge briefly prior to opening vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1140
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Thrombospondin 2 in AsPC-1 whole cell extract and conditioned medium (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with Thrombospondin 2 Monoclonal antibody (Product # MA5-46937) using a dilution of 1:10,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Thrombospondin 2 in 293T whole cell extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with Thrombospondin 2 Monoclonal antibody (Product # MA5-46937) using a dilution of 1:300,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA detection of recombinant HEK293 expressed, full-length human thrombospondin 2 protein. Capture: Thrombospondin 2 monoclonal antibody (Product # MA5-46937) (5 µg/mL); Detection: HRP-conjugated Thrombospondin 2 monoclonal antibody (Product # MA5-46953) (1 µg/mL). Please notice that MA5-46953 needs to be conjugated to HRP to function as the detection antibody when paired with MA5-46937.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Thrombospondin 2 in mock and transfected 293T cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 monoclonal antibody (Product # MA5-46937) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Thrombospondin 2 in paraffin-embedded human pancreatic cancer. Samples were incubated in Thrombospondin 2 Monoclonal antibody (Product # MA5-46937). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
