21663-1-AP
antibody from Invitrogen Antibodies
Targeting: RAB3GAP1
KIAA0066, RAB3GAP, RAB3GAP130, WARBM1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 21663-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB3GAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: PGDAGKAGD QLVPDNLKET DKEKGEVGKS WDSWSDSEEE FFECLSDTEE LKGNGQESGK KGGPKEMANL RPEGRLYQHG KLTLLHNGEP LYIPVTQEPA PMTEDLLEEQ SEVLAKLGTS AEGAHLRARM QSACLLSDME SFKAANPGCS LEDFVRWYSP RDYIEEEVID EKGNVVLKGE LSARMKIPSN MWVEAWETAK PIPARRQRRL FDDTREAEKV LHYLAIQKPA DLARHLLPCV IHAAVLKVKE EESLENISSV KKIIKQIISH SSKVLHFPNP EDKKLEEIIH QITNVEALIA RARSLKAKFG TEKCEQEEEK EDLERFVSCL LEQPEVLVTG AGRGHAGRII H (502-851 aa encoded by BC146809)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.28 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RAB3GAP1 antibody 21663-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using RAB3GAP1 antibody 21663-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 21663-1-AP (RAB3GAP1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 21663-1-AP ( RAB3GAP1 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RAB3GAP1 (IP:21663-1-AP, 5ug; Detection:21663-1-AP 1:500) with mouse kidney tissue lysate 6000ug.