PA5-30678

antibody from Invitrogen Antibodies

Targeting: PHF10 BAF45a, FLJ10975, XAP135

Provider product page for PA5-30678

Western blot

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [2]

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Product number: PA5-30678 - Provider product page
Provider: Invitrogen Antibodies
Product name: PHF10 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HeLa, HeLa nuclear extract, mouse colon. Predicted reactivity: Mouse (93%), Rat (94%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.06 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PHF10 protein structure - PA5-30678 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Sennoside A improved intestinal integrity and metabolic endotoxemia in db / db mice. Serum endotoxin (a) and intestinal permeability (c and e) were measured as described in Materials and Methods. Effects of Sennoside A treatment on TLR4, IkB- alpha , and NF- kappa B in the liver and EAT (b). Sennoside A prevented expressions and corrected distribution of colonic tight junction proteins, occludin, and ZO-1 (scale bar: 100 mu m) (d). Western blot results were consistent with immunofluorescence results, showing a dose-dependent inhibition of expressions of the tight junction proteins in colon tissues (f).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 The Three SWI/SNF Complex Assemblies Have Distinct Regulatory Roles for Foxp3 Expression in Treg Cells (A) A diagram showing three different variants of SWI/SNF complexes: BAF, ncBAF, and PBAF. BAF-specific subunits (Arid1a and Dpf1-Dpf3) are colored blue, ncBAF-specific subunits (Brd9, Smarcd1, Gltscr1l, and Gltscr1) are colored orange, and PBAF-specific subunits (Pbrm1, Arid2, Brd7, and Phf10) are colored green. Shared components among complexes are colored gray. Also shown is an immunoprecipitation assay of Arid1a, Brd9, Phf10, and Smarca4 in Treg cells. The co-precipitated proteins were probed for shared subunits (Smarca4, Smarcc1, and Smarcb1), BAF-specific Arid1a, ncBAF-specific Brd9, and PBAF-specific Pbrm1. (B) FACS histogram of Foxp3 expression in Treg cells after sgRNA targeting of the indicated SWI/SNF subunits. (C) MFI of Foxp3 after sgRNA targeting of the indicated SWI/SNF subunits. Data represent mean and standard deviation of biological replicates (n = 3-21). (D) Principal-component analysis of RNA-seq data collected from Treg cells transduced with guides against the indicated SWI/SNF subunits. In cases where two independent guides were used to target a gene, the second guide for targeting the gene is indicated as ""-2."" (E) MFI of Foxp3 expression in Treg cells after treatment with DMSO or 0.16-10 muM dBRD9 for 4 days. Data represent mean +- SD. Statistical analyses were performed using unpaired two-tailed Student's t test (non-significant [ns], p >=