Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- PA5-49648 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Focal Adhesion Protein Vinculin Is Required for Proper Meiotic Progression during Mouse Spermatogenesis.
The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome.
Petrusová J, Havalda R, Flachs P, Venit T, Darášová A, Hůlková L, Sztacho M, Hozák P
Cells 2022 Jun 23;11(13)
Cells 2022 Jun 23;11(13)
The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome.
Yi JJ, Paranjape SR, Walker MP, Choudhury R, Wolter JM, Fragola G, Emanuele MJ, Major MB, Zylka MJ
The Journal of biological chemistry 2017 Jul 28;292(30):12503-12515
The Journal of biological chemistry 2017 Jul 28;292(30):12503-12515
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSMB1 using an All lanes : Anti-PSMB1 Antibody (C-term) (Product # PA5-49648) at 1:1000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSMB1 in mouse NIH-3T3 cell line (lane 1) and mouse bladder tissue (lane 2) lysates (35 µg/lane). Samples probed using PSMB1 Antibody (C-term) (Product # PA5-49648).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PSMB1 in HepG2 cell. Samples were probed with a PSMB1 Antibody (C-term) (Product # PA5-49648) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PSMB1 in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with a PSMB1 Antibody (C-term) (Product # PA5-49648), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PSMB1 in HL-60 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a PSMB1 Antibody (C-term) (Product # PA5-49648). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 VCL associated with proteasome complexes in the area of the full synapse, and its depletion detached the proteasome from the chromosome axes; ( A ) co-localization of VCL and PSMB in the pachytene spermatocytes was not strong. However, from detailed observation, these two proteins might appear to associate at chromosomal axes; ( B ) VCL/PSMB aggregates appeared more frequently together at the SCP3 full synapsis (a) but not when the SCP3 axes were separate (b); ( C ) the best localization pattern of PSMB in the pachytene spermatocyte visualized by STED. The decorating pattern on the axes was not observed in VCL cKO spermatocytes.