690302

antibody from BioLegend

Targeting: EIF2A EIF-2A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

690302

Provider

BioLegend

Proper citation

BioLegend Cat#690302, RRID:AB_2629818

Product name

Purified anti-eIF-2A

Antibody type

Monoclonal

Antigen

Purified recombinant fragment of human eIF-2A (448-576 a.a.) expressed in E. coli.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P83A7A8

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell or tissue lysates (15 µg protein) from SH-SY5Y, HepG2, OVCAR3 and mouse brain were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-eIF-2A antibody (clone P83A7A8). Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.

Conjugate

Unconjugated