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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-31725 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EIF2A Monoclonal Antibody (3A7A8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3A7A8
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF2a in MCF-7 (1), PC-12 (2), HepG2 (3), HeLa (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate. Samples were incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF2a in human EIF2A recombinant protein. Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF2a in HEK293 (1) and eIF2a cell lysate. Samples were incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of eIF2a in HepG2 cells (green). Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF2a in paraffin-embedded cervical cancer tissue. Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:200-1:1000 followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of eIF2a in HepG2 cells (green). Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:200-1:400 followed by negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of eIF2a in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:10,000.
