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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab101265 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant eIF2A Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human eIF2A Antibody, Recombinant, could be used for WB and ICC/IF.Application:WB: 1/500 Ð 1/1000ICC/IF: 1/50 Ð 1/200Protein FunctionFunctions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 480-494- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant eIF2A Antibody (Ab101265) - Western Blot All lanes: Recombinant eIF2A Antibody (Ab101265) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 65&55 kDa Exposure time: 1.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant eIF2A Antibody (Ab101265) - IF Immunocytochemistry analysis of eIF2A (green) in MCF7 using Recombinant eIF2A Antibody (Ab101265) and cells were counterstained with DAPI (blue).
