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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-15887 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKN2 Monoclonal Antibody (1D1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15887 targets PRK2 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples. The MA5-15887 immunogen is purified recombinant fragment of human PRK2 expressed in E. Coli. . MA5-15887 detects PRK2 which has a predicted molecular weight of approximately 140kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D1
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PKN2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PKN2 Monoclonal Antibody (1D1) (Product # MA5-15887) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using PRK2 monoclonal antibody (Product # MA5-15887) (blue) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using PRK2 monoclonal antibody (Product # MA5-15887) (blue) and negative control (red).
