PA5-121195

antibody from Invitrogen Antibodies

Targeting: GHRHR

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121195

Provider

Invitrogen Antibodies

Product name

GHRHR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: U-251MG, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, Multi-pass membrane protein. Immunogen sequence: MDRRMWGAHV FCVLSPLPTV LGHMHPECDF ITQLREDESA CLQAAEEMPN TTLGCPATWD GLLCWPTAGS GEWVTLPCPD FFSHFSSESG AVKRDCTITG WSEPFPPYPV ACPVPLELLA EEESYFSTVK

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.56 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GHRHR in various lysates. Samples were incubated with GHRHR Polyclonal antibody (Product # PA5-121195) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GHRHR in rat pituitary gland cells. Samples were incubated with GHRHR Polyclonal antibody (Product # PA5-121195) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.