MA5-29382

antibody from Invitrogen Antibodies

Targeting: KYNU

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29382

Provider

Invitrogen Antibodies

Product name

KYNU Recombinant Rabbit Monoclonal Antibody (062)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human KYNU.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

62

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KYNU in Lane A: A549 Whole Cell Lysate (30 µg). Samples were probed using a KYNU Monoclonal Antibody (Product # MA5-29382) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:52 kDa. Observed band size:52 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using KYNU Recombinant Rabbit Monoclonal Antibody (62) (Product # MA5-29382) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 52 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of KYNU in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with KYNU Recombinant Rabbit Monoclonal Antibody (62) (Product # MA5-29382, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

KYNU Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 0.5 µL with KYNU Recombinant Rabbit Monoclonal Antibody (62) (Product # MA5-29382) and 15 µL of 50 % Protein G agarose. Primary antibody: KYNU Recombinant Rabbit Monoclonal Antibody (62), at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 52 kDa.