Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- STJ114963 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-GBP2 antibody (410-520) (STJ114963)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-GBP2 (410-520) is suitable for use in Western Blot and Immunofluorescence.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store in a freezer at-20°C and avoid freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using GBP2 antibody (STJ114963) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of 293T cells, using GBP2 antibody (STJ114963) at 1:1000 dilution. 293T cells treated with different concentrations of IFNA1. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of L929 cells using GBP2 rabbit polyclonal antibody (STJ114963) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA