Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [10]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-47768 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47768, RRID:AB_10010738
- Product name
- Mouse Monoclonal GBP2 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Molecular Subsetting of Interferon Pathways in Sjögren's Syndrome.
Precise probes of type II interferon activity define the origin of interferon signatures in target tissues in rheumatic diseases.
Hall JC, Baer AN, Shah AA, Criswell LA, Shiboski CH, Rosen A, Casciola-Rosen L
Arthritis & rheumatology (Hoboken, N.J.) 2015 Sep;67(9):2437-46
Arthritis & rheumatology (Hoboken, N.J.) 2015 Sep;67(9):2437-46
Precise probes of type II interferon activity define the origin of interferon signatures in target tissues in rheumatic diseases.
Hall JC, Casciola-Rosen L, Berger AE, Kapsogeorgou EK, Cheadle C, Tzioufas AG, Baer AN, Rosen A
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17609-14
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17609-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Kidney tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Ovary tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human prostate tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human thyroid tissue using anti-GBP2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody, and then analyzed by flow cytometry.