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LearnAF3625
antibody from Novus Biologicals
Targeting: IL33
C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF3625 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-33 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) IL-33 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.
Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.
IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C.
Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas.
Dysregulated invertebrate tropomyosin-dectin-1 interaction confers susceptibility to allergic diseases.
Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis.
TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells.
IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.
The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis.
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.
Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts.
Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis.
Zhou Q, Wu X, Wang X, Yu Z, Pan T, Li Z, Chang X, Jin Z, Li J, Zhu Z, Liu B, Su L
Oncogene 2020 Feb;39(7):1414-1428
Oncogene 2020 Feb;39(7):1414-1428
Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.
Izadi D, Layton TB, Williams L, McCann F, Cabrita M, Espirito Santo AI, Xie W, Fritzsche M, Colin-York H, Feldmann M, Midwood KS, Nanchahal J
Science advances 2019 Dec;5(12):eaay0370
Science advances 2019 Dec;5(12):eaay0370
IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C.
Zhang JF, Tao T, Wang K, Zhang GX, Yan Y, Lin HR, Li Y, Guan MW, Yu JJ, Wang XD
Scientific reports 2019 Dec 30;9(1):20276
Scientific reports 2019 Dec 30;9(1):20276
Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas.
Cui G, Ren J, Xu G, Li Z, Zheng W, Yuan A
Cancer cell international 2018;18:203
Cancer cell international 2018;18:203
Dysregulated invertebrate tropomyosin-dectin-1 interaction confers susceptibility to allergic diseases.
Gour N, Lajoie S, Smole U, White M, Hu D, Goddard P, Huntsman S, Eng C, Mak A, Oh S, Kim JH, Sharma A, Plante S, Salem IH, Resch Y, Xiao X, Yao N, Singh A, Vrtala S, Chakir J, Burchard EG, Lane AP, Wills-Karp M
Science immunology 2018 Feb 23;3(20)
Science immunology 2018 Feb 23;3(20)
Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis.
Lapérine O, Cloitre A, Caillon J, Huck O, Bugueno IM, Pilet P, Sourice S, Le Tilly E, Palmer G, Davideau JL, Geoffroy V, Guicheux J, Beck-Cormier S, Lesclous P
PloS one 2016;11(12):e0168080
PloS one 2016;11(12):e0168080
TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells.
Ganesan S, Pham D, Jing Y, Farazuddin M, Hudy MH, Unger B, Comstock AT, Proud D, Lauring AS, Sajjan US
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2409-20
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2409-20
IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.
Mager LF, Riether C, Schürch CM, Banz Y, Wasmer MH, Stuber R, Theocharides AP, Li X, Xia Y, Saito H, Nakae S, Baerlocher GM, Manz MG, McCoy KD, Macpherson AJ, Ochsenbein AF, Beutler B, Krebs P
The Journal of clinical investigation 2015 Jul 1;125(7):2579-91
The Journal of clinical investigation 2015 Jul 1;125(7):2579-91
The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis.
Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Feb;42(2):218-28
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Feb;42(2):218-28
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
Hong YS, Moon SJ, Joo YB, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Park SH, Kim HY, Min JK
Journal of Korean medical science 2011 Sep;26(9):1132-9
Journal of Korean medical science 2011 Sep;26(9):1132-9
The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.
Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM
The American journal of pathology 2011 Jul;179(1):211-22
The American journal of pathology 2011 Jul;179(1):211-22
Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts.
Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y
Gut 2010 Apr;59(4):531-41
Gut 2010 Apr;59(4):531-41
Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis.
Palmer G, Talabot-Ayer D, Lamacchia C, Toy D, Seemayer CA, Viatte S, Finckh A, Smith DE, Gabay C
Arthritis and rheumatism 2009 Mar;60(3):738-49
Arthritis and rheumatism 2009 Mar;60(3):738-49
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-33 in Human Tonsil. IL-33 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-33 and Neutralization by Human IL-33 Antibody. In the presence of sub-optimal amounts of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), Recom-binant Human IL-33 (Catalog # 3625-IL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human IL-33 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625). The ND50 is typically 0.75-3.0 µg/mL.
