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LearnAF5010
antibody from Novus Biologicals
Targeting: IL33
C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- AF5010 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal IL-33 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-33 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with mature recombinant mouse IL-33 and recombinant human IL-33 Propeptide is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IL-33 Propeptide by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse lactating mammary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IL-33 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5010) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-33 Propeptide at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
