LS-C344883

antibody from LSBio

Targeting: IL33 C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C344883

Provider

LSBio

Product name

IL-33 Antibody (clone IL33305B) LS-C344883

Antibody type

Monoclonal

Description

Protein G purified from tissue culture supernatant

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

IL33305B

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human), mAb (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) at 1:2,000 dilution. 1). Recombinant human IL-33 (His tagged). . 2). Recombinant human IL-33 (FLAG-tagged). . 3). Recombinant mouse IL-33 (FLAG-tagged). . 4). Other protein (His tagged, negative control).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical staining of human tissue using anti-IL-33 (human), mAb (IL33305B) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown color). B. Isotype control with IgG2a (negative control).