AF3626

antibody from R&D Systems

Targeting: IL33 C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3626

Provider

R&D Systems

Product name

Mouse IL-33 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL-33 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q8BVZ5


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IL-33 induction and signaling are controlled by glutaredoxin-1 in mouse macrophages.

Weinberg EO, Ferran B, Tsukahara Y, Hatch MMS, Han J, Murdoch CE, Matsui R
PloS one 2019;14(1):e0210827

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RIPK1 prevents TRADD-driven, but TNFR1 independent, apoptosis during development.

Anderton H, Bandala-Sanchez E, Simpson DS, Rickard JA, Ng AP, Di Rago L, Hall C, Vince JE, Silke J, Liccardi G, Feltham R
Cell death and differentiation 2019 May;26(5):877-889

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Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms.

Yamaguchi M, Samuchiwal SK, Quehenberger O, Boyce JA, Balestrieri B
Mucosal immunology 2018 May;11(3):615-626

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IL-1β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation.

Mahmutovic Persson I, Menzel M, Ramu S, Cerps S, Akbarshahi H, Uller L
Respiratory research 2018 Jan 24;19(1):16

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Type 2 Cysteinyl Leukotriene Receptors Drive IL-33-Dependent Type 2 Immunopathology and Aspirin Sensitivity.

Liu T, Barrett NA, Kanaoka Y, Yoshimoto E, Garofalo D, Cirka H, Feng C, Boyce JA
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 1;200(3):915-927

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IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.

Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972

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Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis.

Carrière V, Arshad MI, Le Seyec J, Lefevre B, Farooq M, Jan A, Manuel C, Touami-Bernard L, Lucas-Clerc C, Genet V, Gascan H, Girard JP, Chalmel F, Lamontagne L, Piquet-Pellorce C, Samson M
Mediators of inflammation 2017;2017:1359064

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Cyclic stretch induced IL-33 production through HMGB1/TLR-4 signaling pathway in murine respiratory epithelial cells.

Chang J, Xia Y, Wasserloos K, Deng M, Blose KJ, Vorp DA, Turnquist HR, Billiar TR, Pitt BA, Zhang MZ, Zhang LM
PloS one 2017;12(9):e0184770

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Behavioral Changes in Mice Lacking Interleukin-33.

Dohi E, Choi EY, Rose IVL, Murata AS, Chow S, Niwa M, Kano SI
eNeuro 2017 Nov-Dec;4(6)

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Biliary epithelial injury-induced regenerative response by IL-33 promotes cholangiocarcinogenesis from peribiliary glands.

Nakagawa H, Suzuki N, Hirata Y, Hikiba Y, Hayakawa Y, Kinoshita H, Ihara S, Uchino K, Nishikawa Y, Ijichi H, Otsuka M, Arita J, Sakamoto Y, Hasegawa K, Kokudo N, Tateishi K, Koike K
Proceedings of the National Academy of Sciences of the United States of America 2017 May 9;114(19):E3806-E3815

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IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis.

Chen S, Chen B, Wen Z, Huang Z, Ye L
Oncotarget 2017 May 16;8(20):32407-32418

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Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection.

Qi F, Bai S, Wang D, Xu L, Hu H, Zeng S, Chai R, Liu B
Molecular immunology 2017 Jul;87:284-292

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IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model.

Xu J, Guardado J, Hoffman R, Xu H, Namas R, Vodovotz Y, Xu L, Ramadan M, Brown J, Turnquist HR, Billiar TR
PLoS medicine 2017 Jul;14(7):e1002365

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A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract.

Oh JE, Oh DS, Jung HE, Lee HK
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):E1188-E1195

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The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice.

Han M, Rajput C, Hong JY, Lei J, Hinde JL, Wu Q, Bentley JK, Hershenson MB
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1308-1318

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Early Postnatal Secondhand Smoke Exposure Disrupts Bacterial Clearance and Abolishes Immune Responses in Muco-Obstructive Lung Disease.

Lewis BW, Sultana R, Sharma R, Noël A, Langohr I, Patial S, Penn AL, Saini Y
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 1;199(3):1170-1183

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IL-33 receptor ST2 regulates the cognitive impairments associated with experimental cerebral malaria.

Reverchon F, Mortaud S, Sivoyon M, Maillet I, Laugeray A, Palomo J, Montécot C, Herzine A, Meme S, Meme W, Erard F, Ryffel B, Menuet A, Quesniaux VFJ
PLoS pathogens 2017 Apr;13(4):e1006322

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Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice.

Sezaki T, Hirata Y, Hagiwara T, Kawamura YI, Okamura T, Takanashi R, Nakano K, Tamura-Nakano M, Burkly LC, Dohi T
World journal of gastroenterology 2017 Apr 7;23(13):2294-2307

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Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells.

Li C, Maillet I, Mackowiak C, Viala C, Di Padova F, Li M, Togbe D, Quesniaux V, Lai Y, Ryffel B
Cell death & disease 2017 Apr 6;8(4):e2735

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Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis.

Lapérine O, Cloitre A, Caillon J, Huck O, Bugueno IM, Pilet P, Sourice S, Le Tilly E, Palmer G, Davideau JL, Geoffroy V, Guicheux J, Beck-Cormier S, Lesclous P
PloS one 2016;11(12):e0168080

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Inflammation elevated IL-33 originating from the lung mediates inflammation in acute lung injury.

Lin SH, Fu J, Wang CJ, Gao F, Feng XY, Liu Q, Cao J, Xu F
Clinical immunology (Orlando, Fla.) 2016 Oct 28;

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Lipopolysaccharide Domains Modulate Urovirulence.

Aguiniga LM, Yaggie RE, Schaeffer AJ, Klumpp DJ
Infection and immunity 2016 Nov;84(11):3131-3140

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IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy.

Liu B, Tai Y, Achanta S, Kaelberer MM, Caceres AI, Shao X, Fang J, Jordt SE
Proceedings of the National Academy of Sciences of the United States of America 2016 Nov 22;113(47):E7572-E7579

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Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33.

Athari SK, Poirier E, Biton J, Semerano L, Hervé R, Raffaillac A, Lemeiter D, Herbelin A, Girard JP, Caux F, Boissier MC, Bessis N
Arthritis research & therapy 2016 Jun 18;18(1):143

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Increased expression of upstream TH2-cytokines in a mouse model of viral-induced asthma exacerbation.

Mahmutovic Persson I, Akbarshahi H, Menzel M, Brandelius A, Uller L
Journal of translational medicine 2016 Feb 16;14:52

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Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.

Liu T, Kanaoka Y, Barrett NA, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw TM, Katz HR, Boyce JA
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3537-45

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Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.

Denney L, Byrne AJ, Shea TJ, Buckley JS, Pease JE, Herledan GM, Walker SA, Gregory LG, Lloyd CM
Immunity 2015 Nov 17;43(5):945-58

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Critical role of IL-33 receptor ST2 in experimental cerebral malaria development.

Palomo J, Reverchon F, Piotet J, Besnard AG, Couturier-Maillard A, Maillet I, Tefit M, Erard F, Mazier D, Ryffel B, Quesniaux VF
European journal of immunology 2015 May;45(5):1354-65

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AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.

Kim LK, Morita R, Kobayashi Y, Eisenbarth SC, Lee CG, Elias J, Eynon EE, Flavell RA
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 2;112(22):E2891-9

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Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.

Sundnes O, Pietka W, Loos T, Sponheim J, Rankin AL, Pflanz S, Bertelsen V, Sitek JC, Hol J, Haraldsen G, Khnykin D
The Journal of investigative dermatology 2015 Jul;135(7):1771-1780

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IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.

Mager LF, Riether C, Schürch CM, Banz Y, Wasmer MH, Stuber R, Theocharides AP, Li X, Xia Y, Saito H, Nakae S, Baerlocher GM, Manz MG, McCoy KD, Macpherson AJ, Ochsenbein AF, Beutler B, Krebs P
The Journal of clinical investigation 2015 Jul 1;125(7):2579-91

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IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.

Bu X, Zhang T, Wang C, Ren T, Wen Z
Journal of translational medicine 2015 Dec 16;13:381

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MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease.

Millar NL, Gilchrist DS, Akbar M, Reilly JH, Kerr SC, Campbell AL, Murrell GAC, Liew FY, Kurowska-Stolarska M, McInnes IB
Nature communications 2015 Apr 10;6:6774

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Alternaria-derived serine protease activity drives IL-33-mediated asthma exacerbations.

Snelgrove RJ, Gregory LG, Peiró T, Akthar S, Campbell GA, Walker SA, Lloyd CM
The Journal of allergy and clinical immunology 2014 Sep;134(3):583-592.e6

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Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.

Palm NW, Rosenstein RK, Yu S, Schenten DD, Florsheim E, Medzhitov R
Immunity 2013 Nov 14;39(5):976-85

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Airway epithelial cells initiate the allergen response through transglutaminase 2 by inducing IL-33 expression and a subsequent Th2 response.

Oh K, Seo MW, Lee GY, Byoun OJ, Kang HR, Cho SH, Lee DS
Respiratory research 2013 Mar 13;14:35

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Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.

Scalfone LK, Nel HJ, Gagliardo LF, Cameron JL, Al-Shokri S, Leifer CA, Fallon PG, Appleton JA
Infection and immunity 2013 Apr;81(4):1354-63

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Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.

Paulissen G, El Hour M, Rocks N, Guéders MM, Bureau F, Foidart JM, Lopez-Otin C, Noel A, Cataldo DD
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 15;189(8):4135-43

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Transcriptional regulation of murine IL-33 by TLR and non-TLR agonists.

Polumuri SK, Jayakar GG, Shirey KA, Roberts ZJ, Perkins DJ, Pitha PM, Vogel SN
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 1;189(1):50-60

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The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.

Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM
The American journal of pathology 2011 Jul;179(1):211-22

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Mast cells as sensors of cell injury through IL-33 recognition.

Enoksson M, Lyberg K, Möller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2523-8

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IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.

Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2372-81

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Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.

Ohno T, Oboki K, Morita H, Kajiwara N, Arae K, Tanaka S, Ikeda M, Iikura M, Akiyama T, Inoue J, Matsumoto K, Sudo K, Azuma M, Okumura K, Kamradt T, Saito H, Nakae S
PloS one 2011 Apr 11;6(4):e18404

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IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis.

Hazlett LD, McClellan SA, Barrett RP, Huang X, Zhang Y, Wu M, van Rooijen N, Szliter E
Investigative ophthalmology & visual science 2010 Mar;51(3):1524-32

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Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages.

Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7890-7

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Induction of IL-33 expression and activity in central nervous system glia.

Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
Journal of leukocyte biology 2008 Sep;84(3):631-43

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse IL-33 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with full length mouse IL-33. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-33 in bEnd.3 Mouse Cell Line. IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-33 in Mouse Spleen. IL-33 was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-33 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 5 hours with 50 ng/mL PMA and 1 μg/mL Ca²⁺ ionomycin then stained with Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Proliferation Induced by IL-33 and Neutralization by Human IL-33 Antibody. Recombinant Mouse IL-33 (Catalog # 3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND₅₀ is typically 10-50 ng/mL.