Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB5010 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-33 Propeptide Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-33 Propeptide in direct ELISAs and Western blots. In Western blots, no cross-reactivity with mature recombinant mouse IL-33, mature recombinant human (rh) IL-33, or the pro region of rhIL-33 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BVZ5
- Isotype
- IgG
- Antibody clone number
- 518017
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.
Ohno T, Oboki K, Morita H, Kajiwara N, Arae K, Tanaka S, Ikeda M, Iikura M, Akiyama T, Inoue J, Matsumoto K, Sudo K, Azuma M, Okumura K, Kamradt T, Saito H, Nakae S
PloS one 2011 Apr 11;6(4):e18404
PloS one 2011 Apr 11;6(4):e18404
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse IL-33 Propeptide by Western Blot. Western blot shows lysates of mouse peripheral lymph node tissue under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Mouse IL-33 Propeptide Monoclonal Antibody (Catalog # MAB5010) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for IL-33 Propeptide was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.