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LearnMA5-16242
antibody from Invitrogen Antibodies
Targeting: IL33
C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [2]
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- Product number
- MA5-16242 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-33 Monoclonal Antibody (6H617)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H617
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-33 using a IL-33 monoclonal antibody (Product # MA5-16242) in A) HUVEC cell lysate at 10 µg/mL; and B) partial recombinant IL-33 (amino acids 112-270) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-33 in HUVEC cell lysate, partial recombinant IL-33 (amino acids 112-270). Samples were incubated in IL-33 monoclonal antibody (Product # MA5-16242) using a dilution of 10 µg/mL; 1 µg/mL followed by a goat anti-mouse Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL-33 in Jurkat cells. Samples were incubated in IL-33 monoclonal (Product # MA5-16242) using a dilution of 5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to FITC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL-33 in 0.5 x 10^6 Jurkat cells. Samples were incubated in IL-33 monoclonal antibody (Product # MA5-16242) and 0.25 µg of mouse IgG1 isotype control using a dilution of 0.25 µg followed by anti-mouse IgG PE conjugated secondary antibody. Isotype control (green) and antibody (Clone 6H617; red).
