14341-1-AP

antibody from Invitrogen Antibodies

Targeting: MAVS Cardif, IPS-1, KIAA1271, VISA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14341-1-AP

Provider

Invitrogen Antibodies

Product name

MAVS/VISA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPFAEDKTY KYICRNFSNF CNVDVVEILP YLPCLTARDQ DRLRATCTLS GNRDTLWHLF NTLQRRPGWV EYFIAALRGC ELVDLADEVA SVYESYQPRT SDRPPDPLEP PSLPAERPGP PTPAAAHSIP YNSCREKEPS YPMPVQETQA PESPGENSEQ ALQTLSPRAI PRNPDGGPLE SSSDLAALSP LTSSGHQEKD TELGSTHTAG ATSSLTPSRG PVSPSVSFQP LARSTPRASR LPGPTGSVVS TGTSFSSSSP GLASAGAAEG KQGAESDQAE PIICSSGAEA PANSLPSKVP TTLMPVNTVA LKVPANPASV STVPSKLPTS SKPPGAVPSN ALTNPAPS (1-347 aa encoded by BC044952)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 14341-1-AP) (MAVS; VISA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MAVS antibody 14341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 14341-1-AP ( MAVS; VISA Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 14341-1-AP ( MAVS; VISA Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MAVS; VISA (IP:14341-1-AP, 3ug; Detection:14341-1-AP 1:1000) with HEK-293 cells lysate 1700ug.