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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- PA5-20348 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAVS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A-20 cell lysate.
- Concentration
- 1 mg/mL
Submitted references The Causal Role of Mitochondrial Dynamics in Regulating Innate Immunity in Diabetes.
Chang YH, Lin HY, Shen FC, Su YJ, Chuang JH, Lin TK, Liou CW, Lin CY, Weng SW, Wang PW
Frontiers in endocrinology 2020;11:445
Frontiers in endocrinology 2020;11:445
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A20 cell lysate using a VISA polyclonal antibody (Product # PA5-20348) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of VISA in A20 cell lysate with MAVS Polyclonal Antibody (Product # PA5-20348) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of VISA in Human Brain cells with MAVS Polyclonal Antibody (Product # PA5-20348) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of VISA in human brain tissue with MAVS Polyclonal Antibody (Product # PA5-20348) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Role of MFN1/MFN2 overexpression on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were overexpressed with GFP, GFP-MFN1, or GFP-MFN2 for 18 h, followed by a 6-h exposure with or without 0.25 mM FA. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Role of MFN1/MFN2 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA or between indicated groups. + p < 0.05 comparison between indicated groups. CON, control; FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Role of DRP1/FIS1 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA. + p < 0.05 or between indicated groups. CON, control; FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Role of DRP1/FIS1 overexpression on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effect of mdivi-1 on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were treated with/without FA for 6 h, followed by 10 muM mdivi-1 for another 24 h. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to mock control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
