PA5-20348

antibody from Invitrogen Antibodies

Targeting: MAVS Cardif, IPS-1, KIAA1271, VISA

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-20348 - Provider product page
Provider: Invitrogen Antibodies
Product name: MAVS Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is A-20 cell lysate. PA5-20348 can be used with blocking peptide PEP-0468.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

MAVS protein structure - PA5-20348 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Role of MFN1/MFN2 overexpression on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were overexpressed with GFP, GFP-MFN1, or GFP-MFN2 for 18 h, followed by a 6-h exposure with or without 0.25 mM FA. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Role of MFN1/MFN2 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA or between indicated groups. + p < 0.05 comparison between indicated groups. CON, control; FA, fatty acid.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Role of DRP1/FIS1 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA. + p < 0.05 or between indicated groups. CON, control; FA, fatty acid.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Role of DRP1/FIS1 overexpression on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Effect of mdivi-1 on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were treated with/without FA for 6 h, followed by 10 muM mdivi-1 for another 24 h. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to mock control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.