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LearnNBP1-97618
antibody from Novus Biologicals
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- NBP1-97618 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97618, RRID:AB_11189002
- Product name
- Rat Monoclonal BAFF/BLyS/TNFSF13B Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TACI-BLyS signaling via B-cell-dendritic cell cooperation is required for naive CD8+ T-cell priming in vivo.
CD45 links the B cell receptor with cell survival and is required for the persistence of germinal centers.
B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.
B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Diaz-de-Durana Y, Mantchev GT, Bram RJ, Franco A
Blood 2006 Jan 15;107(2):594-601
Blood 2006 Jan 15;107(2):594-601
CD45 links the B cell receptor with cell survival and is required for the persistence of germinal centers.
Huntington ND, Xu Y, Puthalakath H, Light A, Willis SN, Strasser A, Tarlinton DM
Nature immunology 2006 Feb;7(2):190-8
Nature immunology 2006 Feb;7(2):190-8
B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.
Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):807-17
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):807-17
B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.
Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):807-17
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):807-17
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Batten M, Fletcher C, Ng LG, Groom J, Wheway J, Laâbi Y, Xin X, Schneider P, Tschopp J, Mackay CR, Mackay F
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Batten M, Fletcher C, Ng LG, Groom J, Wheway J, Laâbi Y, Xin X, Schneider P, Tschopp J, Mackay CR, Mackay F
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (8F10) [NBP1-97618] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse TACI (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50 ul FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1ug/ml of rat anti-mTACI 8F10 MAb. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
