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LearnNBP1-97603
antibody from Novus Biologicals
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
ELISA
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- NBP1-97603 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97603, RRID:AB_11161692
- Product name
- Rat Monoclonal BAFF/BLyS/TNFSF13B Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Batten M, Fletcher C, Ng LG, Groom J, Wheway J, Laâbi Y, Xin X, Schneider P, Tschopp J, Mackay CR, Mackay F
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TNFSF13B Antibody (1C9) [NBP1-97603] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (1C9). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
