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LearnNBP1-97639
antibody from Novus Biologicals
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- NBP1-97639 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97639, RRID:AB_11189154
- Product name
- Rat Monoclonal BAFF/BLyS/TNFSF13B Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Tumor necrosis factor superfamily member (TNFSF) 13 (APRIL) and TNFSF13B (BAFF) downregulate homeostatic immunoglobulin production in the intestines.
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Hashiguchi M, Kashiwakura Y, Kanno Y, Kojima H, Kobata T
Cellular immunology 2018 Jan;323:41-48
Cellular immunology 2018 Jan;323:41-48
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Batten M, Fletcher C, Ng LG, Groom J, Wheway J, Laâbi Y, Xin X, Schneider P, Tschopp J, Mackay CR, Mackay F
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma.
Batten M, Fletcher C, Ng LG, Groom J, Wheway J, Laâbi Y, Xin X, Schneider P, Tschopp J, Mackay CR, Mackay F
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):812-22
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
