366502

antibody from BioLegend

Targeting: TNFSF13B BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

366502

Provider

BioLegend

Proper citation

BioLegend Cat#366502, RRID:AB_2565484

Product name

Purified anti-human CD257 (BAFF, BLYS)

Antibody type

Monoclonal

Antigen

Human CD257 transfected murine cell line.

Description

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

1D6

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CD257 (clone 1D6) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.

Conjugate

Unconjugated