11-9017-42
antibody from Invitrogen Antibodies
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
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Validation data
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- Product number
- 11-9017-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD257 (BAFF, BLyS) Monoclonal Antibody (1D6), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1 (TNF and apoptosis leukocyte-expressed ligand-1), THANK (TNF homologue that activates apoptosis, nuclear factor KappaB and c-Jun NH2-terminal kinase) and zTNF4. BLyS, a member of the TNF family, is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes lineage is regulated by IFN-gamma. BLyS acts as a potent B cell growth factor and costimulator of Ig production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease characterized by the presence of autoantibodies against nuclear antigens and immune complexes deposited in the kidney. Data from animal models imply a role for BLyS in human autoimmune diseases. To date BCMA, TACI and BR3 have been identified as receptors for BLyS. APRIL, another TNF family member, and BLyS can form active heterotrimeric molecules when coexpressed and these circulating heterotrimers are present in serum samples from patients with systemic immune-based rheumatic diseases. Furthermore, it has been reported that BLyS and APRIL, share BCMA and TACI as their receptors. Applications Reported: The 1D6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1D6 antibody has been pre-titrated and tested by flow cytometric analysis of human BLyS transfected cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 1D6
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Positive and negative cooperativity of TNF and Interferon-γ in regulating synovial fibroblast function and B cell survival in fibroblast/B cell co-cultures.
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
A functional receptor for B-cell-activating factor is expressed on human acute lymphoblastic leukemias.
Lowin T, Anssar TM, Bäuml M, Classen T, Schneider M, Pongratz G
Scientific reports 2020 Jan 21;10(1):780
Scientific reports 2020 Jan 21;10(1):780
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
Bornacelly A, Mercado D, Acevedo N, Caraballo L
BMC immunology 2014 Jun 7;15:22
BMC immunology 2014 Jun 7;15:22
A functional receptor for B-cell-activating factor is expressed on human acute lymphoblastic leukemias.
Parameswaran R, Müschen M, Kim YM, Groffen J, Heisterkamp N
Cancer research 2010 Jun 1;70(11):4346-56
Cancer research 2010 Jun 1;70(11):4346-56
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human BLyS transfected cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD257 (BAFF, BLyS) FITC (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Cell surface levels of membrane-bound BAFF ( A ), CD40L ( B ), IFN-gammaR ( C ), ObR ( D ), Fn14 ( E ), TACI ( F ), BAFF-R ( G ) and BCMA ( H ) after stimulation with given cytokines for 72 h. The upper panels depict exemplary histograms after flow cytometric analyses. All FACS measurements were normalized to the geometric mean of the unstimulated control value, which was set at 100% (dotted line). P-values are given in the graph. The general linear model with Bonferroni correction was used for all comparisons versus control.
- Conjugate
- Green dye