14-9017-82

antibody from Invitrogen Antibodies
Targeting: TNFSF13B BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Provider product page for 14-9017-82
Western blot
Immunohistochemistry
Flow cytometry
Other assay

Antibody data

Product number
14-9017-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD257 (BAFF, BLyS) Monoclonal Antibody (1D6), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1 (TNF and apoptosis leukocyte-expressed ligand-1), THANK (TNF homologue that activates apoptosis, nuclear factor KappaB and c-Jun NH2-terminal kinase) and zTNF4. BLyS, a member of the TNF family, is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes lineage is regulated by IFN-gamma. BLyS acts as a potent B cell growth factor and costimulator of Ig production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease characterized by the presence of autoantibodies against nuclear antigens and immune complexes deposited in the kidney. Data from animal models imply a role for BLyS in human autoimmune diseases. To date BCMA, TACI and BR3 have been identified as receptors for BLyS. APRIL, another TNF family member, and BLyS can form active heterotrimeric molecules when coexpressed and these circulating heterotrimers are present in serum samples from patients with systemic immune-based rheumatic diseases. Furthermore, it has been reported that BLyS and APRIL, share BCMA and TACI as their receptors. Applications Reported: This 1D6 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining. Applications Tested: This 1D6 antibody has been tested by flow cytometric analysis of human BLlyS transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
1D6
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
TNFSF13B protein structure - 14-9017-82 shown in red.