14-9017-82
antibody from Invitrogen Antibodies
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Other assay [4]
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- Product number
- 14-9017-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD257 (BAFF, BLyS) Monoclonal Antibody (1D6), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1 (TNF and apoptosis leukocyte-expressed ligand-1), THANK (TNF homologue that activates apoptosis, nuclear factor KappaB and c-Jun NH2-terminal kinase) and zTNF4. BLyS, a member of the TNF family, is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes lineage is regulated by IFN-gamma. BLyS acts as a potent B cell growth factor and costimulator of Ig production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease characterized by the presence of autoantibodies against nuclear antigens and immune complexes deposited in the kidney. Data from animal models imply a role for BLyS in human autoimmune diseases. To date BCMA, TACI and BR3 have been identified as receptors for BLyS. APRIL, another TNF family member, and BLyS can form active heterotrimeric molecules when coexpressed and these circulating heterotrimers are present in serum samples from patients with systemic immune-based rheumatic diseases. Furthermore, it has been reported that BLyS and APRIL, share BCMA and TACI as their receptors. Applications Reported: This 1D6 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining. Applications Tested: This 1D6 antibody has been tested by flow cytometric analysis of human BLlyS transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Positive and negative cooperativity of TNF and Interferon-γ in regulating synovial fibroblast function and B cell survival in fibroblast/B cell co-cultures.
Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
A functional receptor for B-cell-activating factor is expressed on human acute lymphoblastic leukemias.
BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex.
BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2.
BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases.
Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators.
Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency.
Lowin T, Anssar TM, Bäuml M, Classen T, Schneider M, Pongratz G
Scientific reports 2020 Jan 21;10(1):780
Scientific reports 2020 Jan 21;10(1):780
Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.
Gideon HP, Phuah J, Junecko BA, Mattila JT
Mucosal immunology 2019 Nov;12(6):1370-1381
Mucosal immunology 2019 Nov;12(6):1370-1381
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
Bornacelly A, Mercado D, Acevedo N, Caraballo L
BMC immunology 2014 Jun 7;15:22
BMC immunology 2014 Jun 7;15:22
A functional receptor for B-cell-activating factor is expressed on human acute lymphoblastic leukemias.
Parameswaran R, Müschen M, Kim YM, Groffen J, Heisterkamp N
Cancer research 2010 Jun 1;70(11):4346-56
Cancer research 2010 Jun 1;70(11):4346-56
BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex.
Hase H, Kanno Y, Kojima M, Hasegawa K, Sakurai D, Kojima H, Tsuchiya N, Tokunaga K, Masawa N, Azuma M, Okumura K, Kobata T
Blood 2004 Mar 15;103(6):2257-65
Blood 2004 Mar 15;103(6):2257-65
BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2.
Kayagaki N, Yan M, Seshasayee D, Wang H, Lee W, French DM, Grewal IS, Cochran AG, Gordon NC, Yin J, Starovasnik MA, Dixit VM
Immunity 2002 Oct;17(4):515-24
Immunity 2002 Oct;17(4):515-24
BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases.
Roschke V, Sosnovtseva S, Ward CD, Hong JS, Smith R, Albert V, Stohl W, Baker KP, Ullrich S, Nardelli B, Hilbert DM, Migone TS
Journal of immunology (Baltimore, Md. : 1950) 2002 Oct 15;169(8):4314-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Oct 15;169(8):4314-21
Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators.
Hsu BL, Harless SM, Lindsley RC, Hilbert DM, Cancro MP
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):5993-6
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):5993-6
Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency.
Yan M, Brady JR, Chan B, Lee WP, Hsu B, Harless S, Cancro M, Grewal IS, Dixit VM
Current biology : CB 2001 Oct 2;11(19):1547-52
Current biology : CB 2001 Oct 2;11(19):1547-52
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Supportive validation
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- Figure 1 Cell surface levels of membrane-bound BAFF ( A ), CD40L ( B ), IFN-gammaR ( C ), ObR ( D ), Fn14 ( E ), TACI ( F ), BAFF-R ( G ) and BCMA ( H ) after stimulation with given cytokines for 72 h. The upper panels depict exemplary histograms after flow cytometric analyses. All FACS measurements were normalized to the geometric mean of the unstimulated control value, which was set at 100% (dotted line). P-values are given in the graph. The general linear model with Bonferroni correction was used for all comparisons versus control.