MA5-29617

antibody from Invitrogen Antibodies

Targeting: TNFSF13B BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29617

Provider

Invitrogen Antibodies

Product name

BAFF Recombinant Rabbit Monoclonal Antibody (182)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human BLyS/TNFSF13B/BAFF.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

182

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BAFF Recombinant Rabbit Monoclonal Antibody (182) (Product # MA5-29617) at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: MOLT-4 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 32 kDa. (We are unsure of the identity of these extra bands).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human BAFF(CD257) expression on U937 cells. Cells were stained with BAFF Recombinant Rabbit Monoclonal Antibody (182) (Product # MA5-29617) (CD257), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BAFF Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 2 µL with BAFF Recombinant Rabbit Monoclonal Antibody (182) (Product # MA5-29617) and 15 µL of 50 % Protein G agarose. Primary antibody: BAFF Recombinant Rabbit Monoclonal Antibody (182), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 31 kDa.