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LearnMA5-29617
antibody from Invitrogen Antibodies
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-29617 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAFF Recombinant Rabbit Monoclonal Antibody (182)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 182
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BAFF Recombinant Rabbit Monoclonal Antibody (182) (Product # MA5-29617) at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: MOLT-4 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 32 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Human BAFF(CD257) expression on U937 cells. Cells were stained with BAFF Recombinant Rabbit Monoclonal Antibody (182) (Product # MA5-29617) (CD257), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BAFF Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 2 µL with BAFF Recombinant Rabbit Monoclonal Antibody (182) (Product # MA5-29617) and 15 µL of 50 % Protein G agarose. Primary antibody: BAFF Recombinant Rabbit Monoclonal Antibody (182), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 31 kDa.
