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antibody from R&D Systems
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF124 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BAFF/BLyS/TNFSF13B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF and less than 1% cross-reactivity with recombinant human (rh) APRIL is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y275- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IFN-γ stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways.
Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype.
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease.
The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis.
BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
Unusual presentation of multiple pathologic bone fractures in a patient with gastric mucosa-associated lymphoid tissue lymphoma.
Essential role of antigen-presenting cell-derived BAFF for antibody responses.
B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
Imbrechts M, De Samblancx K, Fierens K, Brisse E, Vandenhaute J, Mitera T, Libert C, Smets I, Goris A, Wouters C, Matthys P
European journal of immunology 2018 Sep;48(9):1506-1521
European journal of immunology 2018 Sep;48(9):1506-1521
Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype.
Berggren O, Hagberg N, Alexsson A, Weber G, Rönnblom L, Eloranta ML
PloS one 2017;12(8):e0183946
PloS one 2017;12(8):e0183946
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Saulep-Easton D, Vincent FB, Quah PS, Wei A, Ting SB, Croce CM, Tam C, Mackay F
Leukemia 2016 Jan;30(1):163-72
Leukemia 2016 Jan;30(1):163-72
Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease.
Hamada T, Samukawa T, Kumamoto T, Hatanaka K, Tsukuya G, Yamamoto M, Machida K, Watanabe M, Mizuno K, Higashimoto I, Inoue Y, Inoue H
BMC pulmonary medicine 2015 Sep 30;15:110
BMC pulmonary medicine 2015 Sep 30;15:110
The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis.
Abe T, AlSarhan M, Benakanakere MR, Maekawa T, Kinane DF, Cancro MP, Korostoff JM, Hajishengallis G
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1427-35
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1427-35
BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
Zhu J, Sun L, Lin S, Zhao R, Zhou L, Fang D, Chen L, Liu J, Shi W, Zhang L, Yuan S
Journal of experimental & clinical cancer research : CR 2012 Mar 31;31:31
Journal of experimental & clinical cancer research : CR 2012 Mar 31;31:31
Unusual presentation of multiple pathologic bone fractures in a patient with gastric mucosa-associated lymphoid tissue lymphoma.
Kuo SH, Yen RF, Lin CW, Chen LT, Tien HF, Cheng AL
Annals of hematology 2010 Apr;89(4):431-6
Annals of hematology 2010 Apr;89(4):431-6
Essential role of antigen-presenting cell-derived BAFF for antibody responses.
Bergamin F, Vincent IE, Summerfield A, McCullough KC
European journal of immunology 2007 Nov;37(11):3122-30
European journal of immunology 2007 Nov;37(11):3122-30
B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM
Blood 2006 Apr 1;107(7):2882-8
Blood 2006 Apr 1;107(7):2882-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BAFF/BLyS/TNFSF13B in Human Spleen. BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody. In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically 3-12 ng/mL.
