Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-98102 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PTX3 using a PTX3 Polyclonal antibody (Product # PA5-98102) at a concentration of 2 µg/mL. Positive WB detected in: K562 whole cell lysate, Rat lung tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PTX3 Polyclonal Antibody (Product # PA5-98102) and a 42 kDa band corresponding to Pituitary homeobox 3 was observed in HUVEC cell line upon treatment with LPS and Berefeldin but not in HaCaT or BeWo cell lines. Whole cell extracts (40 µg lysate) of HUVEC (Lane 1), HUVEC treated with LPS (0.5 µg/mL for 4h) followed by Brefeldin (300 ng/mL for 20h) (Lane 2), HaCaT (Lane 3), BeWo (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10,000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PTX3 in HepG2 cells using a PTX3 polyclonal antibody (Product # PA5-98102) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PTX3 in paraffin embedded human colon cancer using a PTX3 polyclonal antibody (Product # PA5-98102) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PTX3 in paraffin embedded human lung cancer using a PTX3 polyclonal antibody (Product # PA5-98102) at a dilution of 1:100.