PAB0262
antibody from Abnova Corporation
Targeting: NLRP1
CARD7, CLR17.1, DEFCAP, DKFZp586O1822, KIAA0926, NAC, NALP1, SLEV1, VAMAS1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PAB0262 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0262, RRID:AB_1019065
- Product name
- NLRP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NLRP1.
- Antigen sequence
PSSPDHESPSQESPNAPTST
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NLRP1 (NAC/CARD7) in adult human tissue lysates. Using NLRP1 polyclonal antibody (Cat # PAB0262) at 1 : 2000. Full-length NAC protein was detected at highest levels in kidney, brain and epidermis. A smaller band was detected in thymus, possibly representing a NLRP1 breakdown product or alternatively spliced form. 293T + NLRP1 : positive control, 293T cells transiently transfected with full-length NLRP1.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded normal human tissue sections stained for NLRP1 (CARD7/NAC) using NLRP1 polyclonal antibody (Cat # PAB0262) at 1 : 2000. A, epidermis. B, esophagus. C, uterine cervix. D, thymus. Hematoxylin-eosin counterstain. In A-C, NALP1 expression is associated with differentiation in stratified epithelial of the skin, esophagus, and cervix.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tissue sections stained for NLRP1 (NAC/CARD7) using using NLRP1 polyclonal antibody (Cat # PAB0262) at 1:2000.A, bone marrow.B, brain glioma core from a brain cancer microarray.A1 and B1 are higher magnifications of A and B, respectively. Hemaxotylin-eosin counterstain.
- Validation comment
- Immunohistochemistry