Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP2-22285 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RPL5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPL5 Antibody [NBP2-22285] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22285 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RPL5 Antibody [NBP2-22285] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22285 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RPL5, NBP2-22285 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by western blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody NBP2-22285 used for IP at 3 ug per reaction. RPL5 was also immunoprecipitated by another rabbit anti-RPL5 antibody. For blotting immunoprecipitated RPL5, NBP2-22285 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPL5 Antibody [NBP2-22285] - Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- RPL5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPL5 Antibody [NBP2-22285] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RPL5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (NBP2-22285). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (NBP2-22285). Detection: DAB