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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP9261a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP9261a, RRID:AB_10614129
- Product name
- RPL5 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of RPL5 Antibody (N-term) (Cat. #AP9261a) in Y79 cell line lysates (35ug/lane). RPL5 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- RPL5 Antibody (N-term) (Cat. #AP9261a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
