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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-27539 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, Rat2. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (84%), Pig (97%), Rabbit (98%), Chicken (90%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A single synonymous mutation determines the phosphorylation and stability of the nascent protein.
Karakostis K, Vadivel Gnanasundram S, López I, Thermou A, Wang L, Nylander K, Olivares-Illana V, Fåhraeus R
Journal of molecular cell biology 2019 Mar 1;11(3):187-199
Journal of molecular cell biology 2019 Mar 1;11(3):187-199
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RPL5 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 Polyclonal Antibody (Product # PA5-27539) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
