Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B1455 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B1455, RRID:AB_2179984
- Product name
- IHC-plus™ RIF1 Antibody (aa2375-aa2419) LS-B1455
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Rif1 Antibody - Western Blot. Western blot of Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.