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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C287449 - Provider product page
- Provider
- LSBio
- Product name
- Anti-RIF1 Antibody (aa1425-1450) LS-C287449
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Detection of Human RIF1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RIF1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RIF1 was also immunoprecipitated by the previous lot (-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
