LS-C287450

antibody from LSBio

Targeting: RIF1 FLJ10599, FLJ12870

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C287450

Provider

LSBio

Product name

Anti-RIF1 Antibody (C-Terminus) LS-C287450

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human RIF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RIF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human RIF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RIF1 antibody used for IP at 6 ug/mg lysate. RIF1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RIF1, -4 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 ( Lot4 - left and Lot 3 - right) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.