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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- R35695 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RIF1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific RIF1 antibody is suitable for use in Immunofluorescence/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the RIF1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa and mouse D3 (ES) lysates with RIF1 antibody at 1ug/ml. Predicted molecular weight ~274kDa. Addition of the siRNA reduces RIF1 signal. Tubulin used as a loading control.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human HEK293 cells with RIF1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human U-2 OS cells with RIF1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized mouse NIH 3T3 cells with RIF1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
