Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-1586 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1586, RRID:AB_2179972
- Product name
- Rabbit Polyclonal Rif1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Rif1 regulates the replication timing domains on the human genome.
Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription.
Transcription regulates telomere dynamics in human cancer cells.
Yamazaki S, Ishii A, Kanoh Y, Oda M, Nishito Y, Masai H
The EMBO journal 2012 Sep 12;31(18):3667-77
The EMBO journal 2012 Sep 12;31(18):3667-77
Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription.
Tsai YC, Greco TM, Boonmee A, Miteva Y, Cristea IM
Molecular & cellular proteomics : MCP 2012 May;11(5):60-76
Molecular & cellular proteomics : MCP 2012 May;11(5):60-76
Transcription regulates telomere dynamics in human cancer cells.
Arora R, Brun CM, Azzalin CM
RNA (New York, N.Y.) 2012 Apr;18(4):684-93
RNA (New York, N.Y.) 2012 Apr;18(4):684-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rif1 Antibody [NB100-1586] - Detection of Human RIF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RIF1 antibody NB100-1586 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Rif1 Antibody [NB100-1586] - Whole cell lysate from HeLa cells. Whole cell lysate from 293T cells. Affinity purified rabbit anti-RIF1 antibody NB100-1586. RIF1 was also IPed using rabbit anti-RIF1 antibody NB100-1587.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1586] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).