Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NB100-1587 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1587, RRID:AB_2179971
- Product name
- Rabbit Polyclonal Rif1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rif1 Antibody [NB100-1587] - Whole cell lysate from 293T cells. RIF1 was also immunoprecipitated using rabbit anti-RIF1 antibody NB100-1586.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rif1 Antibody [NB100-1587] - Whole cell lysate (50 ug) from HeLa cells. Antibody used at 0.1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rif1 Antibody [NB100-1587] - Detection of Human Rif1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rif1 antibody NB100-1587 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1587] - Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Rif1 Antibody [NB100-1587] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1587] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.