Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09282PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09282PU-N, RRID:AB_2035918
- Product name
- anti RIF1 (2406-2419)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding amino acids 2406-2419 of Mouse Rif1
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.6 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse R(arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubawith the immunizing peptide (data not shown).Approximately 25 µg of MEF whole cell lysawas separated by SDS-PAGE and transfeonto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h at room temperature followed by washes and reactiowith a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperatuIRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging Systemdeveloped by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.