Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-27402 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The interleukin-20 receptor axis in early rheumatoid arthritis: novel links between disease-associated autoantibodies and radiographic progression.
RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model.
Expression of bone-regulatory proteins in human valve allografts.
Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis.
Kragstrup TW, Greisen SR, Nielsen MA, Rhodes C, Stengaard-Pedersen K, Hetland ML, Hørslev-Petersen K, Junker P, Østergaard M, Hvid M, Vorup-Jensen T, Robinson WH, Sokolove J, Deleuran B
Arthritis research & therapy 2016 Mar 11;18:61
Arthritis research & therapy 2016 Mar 11;18:61
RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model.
Walker CG, Ito Y, Dangaria S, Luan X, Diekwisch TG
European journal of oral sciences 2008 Aug;116(4):312-8
European journal of oral sciences 2008 Aug;116(4):312-8
Expression of bone-regulatory proteins in human valve allografts.
Shetty R, Pepin A, Charest A, Perron J, Doyle D, Voisine P, Dagenais F, Pibarot P, Mathieu P
Heart (British Cardiac Society) 2006 Sep;92(9):1303-8
Heart (British Cardiac Society) 2006 Sep;92(9):1303-8
Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis.
Yoneda T, Ishimaru N, Arakaki R, Kobayashi M, Izawa T, Moriyama K, Hayashi Y
Endocrinology 2004 May;145(5):2384-91
Endocrinology 2004 May;145(5):2384-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 647 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis in RAW cells using 2 ug of PE conjugated antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody.