23408-1-AP

antibody from Proteintech Group

Targeting: TNFSF11 CD254, ODF, OPGL, RANKL, TRANCE

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

23408-1-AP

Provider

Proteintech Group

Product name

RANKL antibody

Antibody type

Polyclonal

Description

RANKL antibody (Cat. #23408-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Small nucleolar RNA host gene 5 plays a role in orthodontic tooth movement by inhibiting osteoclast differentiation.

Feng J, Tan A, Li W, Zheng Y
Orthodontics & craniofacial research 2024 Oct;27(5):775-784

---

Epstein-barr virus infections induce aberrant osteoclastogenesis in immune system-humanized NOD/Shi-scid/IL-2RγC(null) mice.

Nagatsuka Y, Iwata M, Nagasawa Y, Tsuzuki H, Kitamura N, Komatsu A, Kawana K, Ito R, Fujiwara S, Nakamura H, Takei M
Biochemical and biophysical research communications 2024 Jun 30;715:149984

---

Role and Therapeutic Potential for Targeting Fibroblast Growth Factor 10/FGFR1 in Relapsed Rheumatoid Arthritis.

Meng X, Chen Z, Li T, Nie Z, Han H, Zhong S, Yin Z, Sun S, Xie J, Shen J, Xu X, Gao C, Ran L, Xu B, Xiang Z, Wang J, Sun P, Xin P, A X, Zhang C, Qiu G, Gao H, Bian Y, Xu M, Cao B, Li F, Zheng L, Zhang X, Xiao L
Arthritis & rheumatology (Hoboken, N.J.) 2024 Jan;76(1):32-47

---

Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics.

Shao X, Tian Y, Liu J, Yan Z, Ding Y, Hao X, Wang D, Shen L, Luo E, Guo XE, Luo P, Luo W, Cai J, Jing D
Nature communications 2024 Jan 30;15(1):890

---

A parathyroid hormone related supramolecular peptide for multi-functionalized osteoregeneration.

Hao Z, Feng Q, Wang Y, Wang Y, Li H, Hu Y, Chen T, Wang J, Chen R, Lv X, Yang Z, Chen J, Guo X, Li J
Bioactive materials 2024 Apr;34:181-203

---

Confirmation of pain-related neuromodulation mechanism of Bushen Zhuangjin Decoction on knee osteoarthritis.

Tan X, Zheng D, Lin Q, Wang L, Zhu Z, Huang Y, Lin J, Zeng Y, Mao M, Yi Z, Liu L, Ma D, Wang J, Li X
Journal of ethnopharmacology 2024 Apr 24;324:117772

---

Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy.

Liu PL, Diao JY, Wang Q, Liu H, Zhang Y, Liang JQ, Zhang F, Liang XJ, Zhao HM
Analytical cellular pathology (Amsterdam) 2023;2023:7573165

---

Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation.

Li J, Zhang J, Xue Q, Liu B, Qin R, Li Y, Qiu Y, Wang R, Goltzman D, Miao D, Yang R
Aging cell 2023 Sep;22(9):e13912

---

Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis.

Jin Y, Zhou BH, Zhao J, Ommati MM, Wang S, Wang HW
Environmental pollution (Barking, Essex : 1987) 2023 Nov 1;336:122407

---

BML-111 inhibits osteoclast differentiation by suppressing the MAPK and NF-κB pathways, alleviating deterioration of the knee joints in a CIA rat model.

Wang L, Su W, Zheng X, Lin W, Lv C, Yang S, Chen B, Zhang C
Cell biology international 2023 May;47(5):954-968

---

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1.

Hu X, Li M, Zhang Y, Sang K, Zhang Y, Li W, Liu B, Wan L, Du B, Qian J, Meng F, Fu Y, Dai M, Gao G, Ye H
Experimental cell research 2023 Mar 1;424(1):113490

---

Effects of green tea extract epigallocatechin-3-gallate (EGCG) on orthodontic tooth movement and root resorption in rats.

Zou JH, Chen F, Li YL, Chen H, Sun TK, Du SM, Zhang J
Archives of oral biology 2023 Jun;150:105691

---

Pulsed frequency modulated ultrasound promotes therapeutic effects of osteoporosis induced by ovarian failure in mice.

Zhou X, Sun S, Chen Y, Liu C, Li D, Cheng Q, He M, Li Y, Xu K, Ta D
Ultrasonics 2023 Jul;132:106973

---

Patellar instability-induced bone loss in the femoral trochlea is associated with the activation of the JAK1/STAT3 signaling pathway in growing mice.

Ma C, Kou W, Cui Z, Liu W, Liu C, Wang S, Wang F
Journal of orthopaedic surgery and research 2023 Jul 24;18(1):526

---

Clearance of senescent cells by navitoclax (ABT263) rejuvenates UHMWPE-induced osteolysis.

Su W, Hu Y, Fan X, Xie J
International immunopharmacology 2023 Feb;115:109694

---

BRG1 is involved in vascular calcification in chronic renal disease via autophagy of vascular smooth muscle cells.

Feng Y, Yu M, Wang C, Xia J, Huang L, Tang Y, Xiao Q, Pu L, Wang L, Li G, Li Y
iScience 2023 Apr 21;26(4):106485

---

S-Equol enhances osteoblastic bone formation and prevents bone loss through OPG/RANKL via the PI3K/Akt pathway in streptozotocin-induced diabetic rats.

Xu Z, Xu J, Li S, Cui H, Zhang G, Ni X, Wang J
Frontiers in nutrition 2022;9:986192

---

Zoledronic acid generates a spatiotemporal effect to attenuate osteoarthritis by inhibiting potential Wnt5a-associated abnormal subchondral bone resorption.

Ding D, Wang L, Yan J, Zhou Y, Feng G, Ma L, Yang Y, Pei X, Jin Q
PloS one 2022;17(7):e0271485

---

Sinomenine Inhibits Orthodontic Tooth Movement and Root Resorption in Rats and Enhances Osteogenic Differentiation of PDLSCs.

Li H, Li Y, Zou J, Yang Y, Han R, Zhang J
Drug design, development and therapy 2022;16:2949-2965

---

Fecal microbiota transplantation ameliorates bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and metabolic function.

Zhang YW, Cao MM, Li YJ, Lu PP, Dai GC, Zhang M, Wang H, Rui YF
Journal of orthopaedic translation 2022 Nov;37:46-60

---

Preconception exposure to dibutyl phthalate (DBP) impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells.

Xie Z, Liu S, Hua S, Wu L, Zhang Y, Zhu Y, Shi F, Jiang J
Toxicology 2022 May 30;474:153213

---

Hexokinase 2-mediated glycolysis promotes receptor activator of NF-κB ligand expression in Porphyromonas gingivalis lipopolysaccharide-treated osteoblasts.

Yu Y, Jiang L, Li J, Lei L, Li H
Journal of periodontology 2022 Jul;93(7):1036-1047

---

Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model.

Lin C, Chen Z, Guo D, Zhou L, Lin S, Li C, Li S, Wang X, Lin B, Ding Y
Aging 2022 Jan 4;14(1):253-271

---

Saponins from Nigella glandulifera seeds attenuate collagen-induced rheumatoid arthritis in rats via the OPG/RANKL/NF-κB and Ang/Tie-2 pathways.

Jiang H, Xu F, Zeng L, Li C, Chen Y, Wang L, Li Z, Liu R
Journal of ethnopharmacology 2022 Jan 30;283:114714

---

Ubiquitin-specific protease 47 is associated with vascular calcification in chronic kidney disease by regulating osteogenic transdifferentiation of vascular smooth muscle cells.

Xiao Q, Tang Y, Xia J, Luo H, Yu M, Chen S, Wang W, Pu L, Wang L, Li G, Li Y
Renal failure 2022 Dec;44(1):752-766

---

Piezo1-mediated fluid shear stress promotes OPG and inhibits RANKL via NOTCH3 in MLO-Y4 osteocytes.

Liu Z, Tang Y, He L, Geng B, Lu F, He J, Yi Q, Liu X, Zhang K, Wang L, Xia Y, Jiang J
Channels (Austin, Tex.) 2022 Dec;16(1):127-136

---

Asperuloside Prevents Peri-Implantitis via Suppression of NF-κB and ERK1/2 on Rats.

Wang X, Chen X, Zhang Z, Chen J, Ge Z, Huang S, Wei H, Li D
Pharmaceuticals (Basel, Switzerland) 2022 Aug 20;15(8)

---

Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE(-/-) mice.

Su R, Jin X, Zhao W, Wu X, Zhai F, Li Z
Ecotoxicology and environmental safety 2022 Apr 1;234:113410

---

Total Saponins from Nigella glandulifera Seeds Ameliorate Adjuvant-Induced Rheumatoid Arthritis in Rats by Inhibition of an Inflammatory Response and Bone Erosion.

Zeng L, Li C, Jiang H, Chen Y, Li Z, Xu F, Liu R
BioMed research international 2021;2021:6613527

---

Metformin attenuates osteoclast-mediated abnormal subchondral bone remodeling and alleviates osteoarthritis via AMPK/NF-κB/ERK signaling pathway.

Guo H, Ding D, Wang L, Yan J, Ma L, Jin Q
PloS one 2021;16(12):e0261127

---

Tomatidine Suppresses the Destructive Behaviors of Fibroblast-Like Synoviocytes and Ameliorates Type II Collagen-Induced Arthritis in Rats.

Yu X, Zhou J, Zhao F, Liu X, Mao Y, Diao L, Wen C, Liu M
Frontiers in pharmacology 2021;12:670707

---

Regulatory roles of miRNAs 16, 133a, and 223 on osteoclastic bone destruction caused by breast cancer metastasis.

Kitayama K, Kawamoto T, Kawakami Y, Hara H, Takemori T, Fujiwara S, Yahiro S, Miyamoto T, Mifune Y, Hoshino Y, Kakutani K, Matsumoto T, Matsushita T, Niikura T, Kuroda R, Akisue T
International journal of oncology 2021 Nov;59(5)

---

Loss of chemerin triggers bone remodeling in vivo and in vitro.

Han L, Zhang Y, Wan S, Wei Q, Shang W, Huang G, Fang P, Min W
Molecular metabolism 2021 Nov;53:101322

---

Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.

Jia X, Zhu H, Li G, Lan M, Li X, Huang M, Xu W, Wu S
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 May;137:111321

---

Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p.

Yu L, Sui B, Fan W, Lei L, Zhou L, Yang L, Diao Y, Zhang Y, Li Z, Liu J, Hao X
Journal of extracellular vesicles 2021 Jan;10(3):e12056

---

Anti-rheumatoid arthritis effects of iridoid glucosides from Lamiophlomis rotata (Benth.) kudo on adjuvant-induced arthritis in rats by OPG/RANKL/NF-κB signaling pathways.

Zhao X, Jiang S, Dong Q, Dang J, Liu Z, Han H, Tao Y, Yue H
Journal of ethnopharmacology 2021 Feb 10;266:113402

---

Milk Polar Lipids Supplementation to Obese Rats During Pregnancy and Lactation Benefited Skeletal Outcomes of Male Offspring.

Han L, Du M, Ren F, Mao X
Molecular nutrition & food research 2021 Aug;65(15):e2001208

---

Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification.

Lin X, Zhu T, Xu F, Zhong JY, Li F, Shan SK, Wu F, Guo B, Zheng MH, Wang Y, Xu QS, Liao XB, Lu HY, Xie XB, Yuan LQ
Frontiers in cell and developmental biology 2020;8:618228

---

Genistein prevents bone loss in type 2 diabetic rats induced by streptozotocin.

Lu R, Zheng Z, Yin Y, Jiang Z
Food & nutrition research 2020;64

---

The effects of binge alcohol exposure on tooth movement and associated root resorption in rats.

Yang F, Wang XX, Li J, Nie FJ, Cui Q, Fu YJ, Zhang J
Alcohol (Fayetteville, N.Y.) 2020 Nov;88:1-9

---

Glyburide inhibits the bone resorption induced by traumatic occlusion in rats.

Arita Y, Yoshinaga Y, Kaneko T, Kawahara Y, Nakamura K, Ohgi K, Arita S, Ryu T, Takase M, Sakagami R
Journal of periodontal research 2020 Jun;55(3):464-471

---

MicroRNA‑21 serves an important role during PAOO‑facilitated orthodontic tooth movement.

Zhang Y, Tian Y, Yang X, Zhao Z, Feng C, Zhang Y
Molecular medicine reports 2020 Jul;22(1):474-482

---

Sialic acid-modified chitosan oligosaccharide-based biphasic calcium phosphate promote synergetic bone formation in rheumatoid arthritis therapy.

Xu XL, Shu GF, Wang XJ, Qi J, Jin FY, Shen QY, Ying XY, Ji JS, Du YZ
Journal of controlled release : official journal of the Controlled Release Society 2020 Jul 10;323:578-590

---

Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease.

Zhong W, Peng Y, Yue E, Huang B, Zhang W, Zhao Z, Jiang J, Wang Q, Zhao H
Oral diseases 2020 Jan;26(1):182-192

---

Fluid Shear Stress Suppresses Osteoclast Differentiation in RAW264.7 Cells through Extracellular Signal-Regulated Kinase 5 (ERK5) Signaling Pathway.

Ma C, Geng B, Zhang X, Li R, Yang X, Xia Y
Medical science monitor : international medical journal of experimental and clinical research 2020 Jan 8;26:e918370

---

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/β-catenin signaling.

Zheng W, Ding B, Li X, Liu D, Yokota H, Zhang P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Feb;34(2):3399-3412

---

Effects Of Triptolide On Tooth Movement And Root Resorption In Rats.

Yang F, Wang XX, Ma D, Cui Q, Zheng H, Liu XC, Zhang J
Drug design, development and therapy 2019;13:3963-3975

---

Elevated ATF4 Expression in Odontogenic Keratocysts Epithelia: Potential Involvement in Tissue Hypoxia and Stromal M2 Macrophage Infiltration.

Zhong WQ, Li ZZ, Jiang H, Zou YP, Wang HT, Cai Y, Zhao Y, Zhao JH
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2019 Nov;67(11):801-812

---

LOX-1 is involved in TLR2 induced RANKL regulation in peri-implantitis.

Zhang Q, Liu J, Ma L, Bai N, Xu H
International immunopharmacology 2019 Dec;77:105956

---

Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts.

Man QW, Zhong WQ, Ren JG, Liu JY, Zheng YY, Li RF, Nyimi BF, Chen G, Zhao YF, Liu B
International journal of oncology 2018 Jun;52(6):1863-1874

---

β-Estradiol antagonizes the inhibitory effects of caffeine in BMMSCs via the ERβ-mediated cAMP-dependent PKA pathway.

Wang C, Zhou Y, Guan X, Yu M, Wang H
Toxicology 2018 Feb 1;394:1-10

---

Role of endoplasmic reticulum stress in disuse osteoporosis.

Li J, Yang S, Li X, Liu D, Wang Z, Guo J, Tan N, Gao Z, Zhao X, Zhang J, Gou F, Yokota H, Zhang P
Bone 2017 Apr;97:2-14

---

Knee loading inhibits osteoclast lineage in a mouse model of osteoarthritis.

Li X, Yang J, Liu D, Li J, Niu K, Feng S, Yokota H, Zhang P
Scientific reports 2016 Apr 18;6:24668

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 23408-1-AP( RANKL Antibody) at dilution of 1:700

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The RANKL antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human RANKL. This antibody recognizes human antigen. The RANKL antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

23408-1-AP.pdf

Show more