TA328429

antibody from OriGene

Targeting: TNFSF11 CD254, ODF, OPGL, RANKL, TRANCE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA328429

Provider

OriGene

Product name

Anti-Human sRANK Ligand Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-Human sRANK Ligand Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

TNFSF11

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

This antibody stained formalin-fixed, paraffin-embedded sections of human metastatic carcinoma of lymph node from breast tissue. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

Validation comment

IHC