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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB6262 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TRANCE/TNFSF11/RANK L in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.
Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.
Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1103-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1103-8
Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.
Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1103-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1103-8
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) activity in cell lysates . Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TRANCE/ TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6262). The ND50 is typically 0.01-0.04 µg/mL.
