14-6619-80

antibody from Invitrogen Antibodies
Targeting: TNFSF11 CD254, ODF, OPGL, RANKL, TRANCE
Provider product page for 14-6619-80

Antibody data

Product number
14-6619-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD254 (RANK Ligand) Monoclonal Antibody (MIH24), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The MIH24 monoclonal antibody reacts with human TRANCE (TNF-related activation-induced cytokine receptor) also known as RANKL (receptor activator of NF-kappaB ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL. Applications Reported: The MIH24 antibody has been reported for use in flow cytometric analysis, and ELISA. Applications Tested: The MIH24 antibody has been tested by flow cytometric analysis of human RANKL transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
MIH24
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
TNFSF11 protein structure - 14-6619-80 shown in red.