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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [2]
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- Product number
- PA5-110268 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RANKL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: RISEDGTHCI YRILRLHENA DFQDTTLESQ DTKLIPDSCR RIKQAFQGAV QKELQHIVGS QHIRAEKAMV DGSWLDLAKR SKLEAQPFAH LTINATDIPS GSHKVSLSSW YHDRGWAKIS NMTFSNGKLI VNQDGFYYLY ANICFRHHET SGDLATEYLQ LMVYVTKTSI KIPSSHTLMK GGSTKYWSGN SEFHFYSINV GGFFKLRSGE EISIEVSNPS LLDPDQDATY FGAFKVRDID
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Combined Therapy of Yishen Zhuanggu Decoction and Caltrate D600 Alleviates Postmenopausal Osteoporosis by Targeting FoxO3a and Activating the Wnt/β-Catenin Pathway.
Involvement of the TNF-α/SATB2 axis in the induced apoptosis and inhibited autophagy of osteoblasts by the antipsychotic Risperidone.
Caspase-1 Inhibition Impacts the Formation of Chondrogenic Nodules, and the Expression of Markers Related to Osteogenic Differentiation and Lipid Metabolism.
Li B, Jiang C, Zhan X
Evidence-based complementary and alternative medicine : eCAM 2022;2022:7732508
Evidence-based complementary and alternative medicine : eCAM 2022;2022:7732508
Involvement of the TNF-α/SATB2 axis in the induced apoptosis and inhibited autophagy of osteoblasts by the antipsychotic Risperidone.
Zhang S, He W, Li A, Zhao C, Chen Y, Xu C, Zhang Q, Zheng D, Chen M, Miao H, Huang Y
Molecular medicine (Cambridge, Mass.) 2022 May 3;28(1):46
Molecular medicine (Cambridge, Mass.) 2022 May 3;28(1):46
Caspase-1 Inhibition Impacts the Formation of Chondrogenic Nodules, and the Expression of Markers Related to Osteogenic Differentiation and Lipid Metabolism.
Ramesova A, Vesela B, Svandova E, Lesot H, Matalova E
International journal of molecular sciences 2021 Sep 3;22(17)
International journal of molecular sciences 2021 Sep 3;22(17)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RANKL in A-549, mouse thymus, mouse lung, rat thymus. Samples were incubated in polyclonal RANKL antibody (Product # PA5-110268) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of RANKL in HeLa cells. Samples were incubated in polyclonal RANKL antibody (Product # PA5-110268) using a dilution of 1:100, followed by DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RANKL in paraffin-embedded mouse Intestine tissue. Samples were incubated in polyclonal RANKL antibody (Product # PA5-110268) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RANKL in paraffin-embedded mouse kidney tissue. Samples were incubated in polyclonal RANKL antibody (Product # PA5-110268) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RANKL in paraffin-embedded rat intestine tissue. Samples were incubated in polyclonal RANKL antibody (Product # PA5-110268) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RANKL in paraffin-embedded rat kidney tissue. Samples were incubated in polyclonal RANKL antibody (Product # PA5-110268) using a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Expression of Rankl and Opg in caspase-1 inhibited or Cd36 silenced micromass cultures. Gene expression of Rankl ( A ) and Opg ( B ) after 6 days of caspase-1 inhibition. Immunofluorescent detection of Rankl (green) in micromass cultures after 6 days of cultivation with caspase-1 inhibitor ( C ) and in control micromasses ( D ). Expression of Cd36 ( E ), Pparg ( F ), Rankl ( G ), and Opg ( H ) after 6 days of Cd36 silencing in micromass cultures. Scale bar = 100 mum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Risperidone confers an inhibitory effect on differentiation and autophagy and a promotive effect on apoptosis of osteoblasts in vitro. A the mRNA expression of OPG, collagen I and RANKL in MC3T3-E1 cells determined by RT-qPCR. B - C Western blot analysis of differentiation-related protein (OPG, collagen I and RANKL) expression in MC3T3-E1 cells. The band intensity was assessed. D representative images of ALP staining in MC3T3-E1 cells. E, statistical results of ALP positive rate in MC3T3-E1 cells. F representative images of alizarin red S staining in MC3T3-E1 cells. G statistical results of integrated optical density (IOD) of alizarin red S in MC3T3-E1 cells. H representative images of autophagosome in MC3T3-E1 cells assessed by immunofluorescence. I Western blot analysis of autophagy-related protein (LC3 II/I, Beclin1, and p62) expression in MC3T3-E1 cells. The band intensity was assessed. J Western blot analysis of apoptosis-related protein (cleaved PARP1, cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9) expression in MC3T3-E1 cells. K apoptosis rate in MC3T3-E1 cells evaluated by flow cytometry. * p < 0.05, compared with MC3T3-E1 cells treated with normal culture medium. The cell experiments are repeated 3 times
