PA5-21951

antibody from Invitrogen Antibodies

Targeting: TNFSF11 CD254, ODF, OPGL, RANKL, TRANCE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21951

Provider

Invitrogen Antibodies

Product name

CD254 (RANK Ligand) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Rat lung.

Concentration

1 mg/mL

References

Submitted references

Co-expression of CD44+/RANKL+ tumor cells in the carcinogenesis of oral squamous cell carcinoma.

Grimm M, Renz C, Munz A, Hoefert S, Krimmel M, Reinert S
Odontology 2015 Jan;103(1):36-49

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD254 (RANK Ligand) Polyclonal Antibody (Product # PA5-21951). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. RANKL (CD254) antibody. CD254 (RANK Ligand) Polyclonal Antibody (Product # PA5-21951) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD254 (RANK Ligand) Polyclonal Antibody detects RANKL protein by western blot analysis. A. 50 µg rat lung extract.10% SDS-PAGE. CD254 (RANK Ligand) Polyclonal Antibody (Product # PA5-21951) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD254 (RANK Ligand) Polyclonal Antibody detects RANKL protein at membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CD254 (RANK Ligand) Polyclonal Antibody (Product # PA5-21951) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.