Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-16156 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD254 (RANK Ligand) Monoclonal Antibody (12A668)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Antibody clone number
- 12A668
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of a partial recombinant Trance protein probed with 2 µg/mL of a CD254/RANKL monoclonal antibody (Product # MA5-16156).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD254 (RANK Ligand) in 35 µg per lane human lymph node lysate. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA5-16156). Band detected at ~35 kDa and ~28 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD254 (RANK Ligand) in transfected cell lysate. Sample was incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA5-16156).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CD254 (RANK Ligand) in immersion fixed mouse splenocytes. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA5-16156). Incubated for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD254 (RANK Ligand) in formalin-fixed paraffin-embedded human liver. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA5-16156) followed by peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD254 (RANK Ligand) in formalin-fixed paraffin-embedded human lymph node. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA5-16156).